Detection of Leishmania DNA in paraffin embedded specimens of chronic lupoid leishmaniasis using polymerase chain reaction
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چکیده
Result: Eleven male and 9 female patients with a mean age of 17.95 years were included in our study. The mean duration of lesions was 3.69 years. Most of the lesions (18 of 20 cases) were located on the face and others were on the limbs. Histologic study showed tuberculoid granulomatous inflammation with a variable ratio of histiocytes and lymphocytes without detection of leishman body. PCR was positive in 12 cases (60% of cases). We could not find any correlation between the results of PCR and sex, age, duration and location of the lesions. Histiocyteprominent lesions were more prone to be positive on PCR examination but that was not statistically significant.
منابع مشابه
Detection of Leishmania DNA in paraffin embedded specimens of chronic lupoid leishmaniasis using polymerase chain reaction
Background: Chronic lupoid leishmaniasis (CLL) is a chronicform of cutaneous leishmaniasis that is usually resistant to antileishmania agents and leishmania is not seen in the histologicsections. We used polymerase chain reaction (PCR) to detectleishmania DNA in CLL specimens.Method: This descriptive cross sectional study was done on20 paraffin embedded specimens of CLL cases referred to theder...
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